黄连化学成分的分离与鉴定

Isolation and identification of chemical constituents from rhizoma of Coptis chinensis

目的研究黄连（CoptischinensisFranch．）根茎的化学成分。方法利用硅胶柱色谱、凝胶柱色谱、中低压液相色谱、高效液相色谱等色谱技术对黄连的化学成分进行分离和纯化，通过NMR等波谱数据分析确定化合物的结构。结果从黄连正丁醇层提取物中分离得到11个已知成分，分别鉴定为降氧化北美黄连次碱（noroxyhydrastineine，1）、3，4-二氢-6，7-二甲氧基异喹诺酮（6，7-di-methoxy-3，4-dihydro-2H-isoquinolin-1-one，2）、8-氧化黄连碱（8-oxocoptisine，3）、小檗碱（berberine，4）、氧化小檗碱（oxyberberine，5）、原儿茶酸甲酯（protocatechuicacidmethylester，6）、丹参素甲正丁酯（n-butyl 3,4-dihydroxyphenyllactate，7）、反式-3，4-二甲氧基肉桂酸（E-3，4-dimethoxycinnamicacid，8）、阿魏酸正丁酯（n-butyl ferulate，9）、5-O-feruloylquinicacidbutylester（10）、4-O—feru—loylquinic acid butyl ester（11）。结论化合物7～11为首次从黄连属植物中分离得到。

Objective To study the chemical constituents of rhizoma of Coptis chinensis. Methods The com- pounds from the n-butanol extracts were isolated by silica gel column, Sephadex LH-20 column chromatography and HPLC techniques. And these compounds were identified by the physico-chemical properties and spectroscopic methods. Results Eleven compounds were isolated from the n-butanol extract of C. chinensis, whose structures were determined as noroxyhydrastineine（ 1 ） ,6,7-dimethoxy-3,4-dihydro-2H-isoquinolin-1- one （ 2 ）, 8-oxocoptisine （ 3 ）, berberine （ 4 ）, oxyberberine （ 5 ）, protocatechuic acid methyl ester （ 6 ）, n -bntyl3, 4-dihydroxyphenyllactate （ 7 ）, E-3,4-dimethoxycinnamic acid （ 8 ）, n-butyl ferulate （ 9 ）, 5-O-feruloylquinic acid butyl ester（10） ,4-O-feruloylquinic acid butyl ester（ 11 ）. Conclusions Compounds 7-11 are isolated from C. chinensis for the first time.