染料木素等5种黄酮类物质对小鼠血清尿酸水平及黄嘌呤氧化酶活性的影响

Effects of genistein in five different flavonoid compounds on xanthine oxidase activities and serum uric acid levels in mice

目的探讨黄酮类化合物染料木素、芹菜素、槲皮素、芦丁和落新妇苷体外对黄嘌呤氧化酶活性的影响,对正常小鼠血清和肝脏黄嘌呤氧化酶活性的影响,同时评价对小鼠血清尿酸水平的作用。方法采用改良的紫外分光光度法测定染料木素、芹菜素、槲皮素、芦丁和落新妇苷体外对黄嘌呤氧化酶的抑制作用,采用分光光度法研究对小鼠血清和肝脏黄嘌呤氧化酶活性的影响,以磷钨酸法测定对小鼠血清尿酸水平的作用。结果体外实验表明黄酮类化合物染料木素、芹菜素、槲皮素、芦丁和落新妇苷体外对黄嘌呤氧化酶活性无明显影响。体内实验观察到这5种黄酮类化合物能够显著升高或降低黄嘌呤氧化酶的活性;而且,血清尿酸水平与血清黄嘌呤氧化酶活性密切相关,与肝脏黄嘌呤氧化酶活性无明显关联。用这些黄酮类化合物给药的小鼠血清尿酸水平都高于正常对照组。结论这5种黄酮类化合物不能够作为替代别嘌醇的药物用来降低血清尿酸水平。

Objective To investigate the effects of the flavonoids genistein, apigenin, quercetin, rutin and astilbin on xanthine oxidase （XO） activities in vitro, and in serum and liver. To measure the effects of the flavonoids on serum uric acid levels in vivo. Methods The inhibitory effects of genistein, apigenin, quercetin, rutin and astilbin on in vitro xanthine oxidase activity were determined using a modified spectrophotometfic method. The XO activity in serum and liver were determined spectrophotometrically using standard diagnostic kits;meanwhile, serum uric acid levels were determined using the phosphotungstic acid method. Results In vitro studies indicated that the flavonoids tested did not significantly affect XO activity. However, signifi- cant increases and decreases in XO activities were observed in vivo. Moreover, serum XO activity was correlated with serum uric acid levels, while no correlation was observed for liver XO activity. The present study showed that serum uric acid levels in mice treated with the flavonoids tested here are higher than control levels. Conclusions The tested flavonoids cannot be the candidates to replace allopurinol as a treatment to reduce serum uric acid levels.