氟酰褪黑素对小鼠免疫功能的影响

Effect of fluorine acyl melatonin on immune functions in mice

目的探讨合成免疫调节剂氟酰褪黑素（N-（2-（5-methoxy-2-ethoxycarbonyl-3-indolyl）ethyl）trifluoroacetamide，TFMMT）对小鼠体内、外免疫活性的调节作用。方法使用正常动物模型，通过碳粒廓清实验、溶血素水平、迟发超敏反应、淋巴细胞转化实验及白介素-2（IL-2）的释放，测定小鼠的免疫功能。结果TFMMT3个剂量组均能在不同程度上增强巨噬细胞的吞噬能力，提高血清溶血素水平；TFMMT高剂量组能提高小鼠对体内迟发超敏反应细胞的功能；浓度在2．03×10^-10～6．98×10^-7mol·L^-1内，TFMMT剂量依赖性使小鼠脾脏中T淋巴细胞对ConA的反应性显著提高，TFMMT可使正常小鼠脾细胞分泌IL-2的水平升高，随剂量不断增大，作用反而减弱，呈剂量依赖性，但以1．17×10^-9 - 6．98×10^-7mol·L^-1内较为显著。结论TFMMT能在体液、细胞及非特异性免疫等不同角度增强小鼠的免疫功能。

Objective To test in vivo and vitro immunoregulatory activity of fluorine acyl melatonin （TFM- MT ） in mice. Methods Normal mice models were used in the experiment. Carbon clearance assay, erythrolysin test, delayed hypersensitive reaction, lymphocyte transformation and IL-2 releasing determination were also introduced to analyze immune function. Results Three doses of TFMMT could enhance the phagocytose activity of macrophage, and significantly increased the contents of serum IgM antibody. The high dose enhanced the delayed hypersensitive reaction. TFMMT enchanced ConA-stimulated transformations of splenic lymphocyte in 2.03×10^-10～6.98×10^-7mol·L^-1. In 1.17×10^-9 - 6.98×10^-7mol·L^-1 ,secretion of IL- 2 in spleen of mice present that low dose had promotion and high dose had decrease. Conclusions TFMMT can regulate immune function of mice in humoral immune,cellule immune and non-specific immune.