弱酸性离子交换树脂纯化黄连总生物碱

Purification of Total Alkaloid from Rhizoma Coptidis by Weakly Acidic Ion Exchange Resin

目的:探寻大孔树脂纯化黄连总生物碱的工艺条件,为规模化生产提供可行性方案。方法:以盐酸小檗碱含量为指标对D101,AB-8,D151等大孔树脂纯化总黄连生物碱的效果进行考察。结果:D151型弱酸性离子交换树脂对总生物碱的吸附能力明显强于其他树脂,适宜的工艺条件为生药质量浓度0.15 g.L-1上样,上样量9 BV,水洗体积2 BV,酸洗浓度为0.12mol.L-1 HCl溶液,用量5 BV。此条件下,所得浸膏量减少60%以上,总生物碱质量分数达80%以上,样品回收率在70%以上。结论:D151型弱酸性离子交换树脂对黄连总生物碱的纯化效果较好,其载药量较大,适合规模化生产。

Objective：To investigate the purification technology of total alkaloids from Rhizoma Coptidis by macroporous resin,and to provide feasible program for large-scale manufacture.Method：Evaluated the effect of purification of total alkaloids from Rhizoma Coptidis by D101,AB-8 and D151 resins respectively with the indicator of the content of berberine.Result：It has been found that D151 resin＇s adsorption capacity of total alkaloids was the best in those three resins.The optimum process was as follows：the concentration of the sample solution was 0.15 g·L-1 with 9 times the amount of resin volume,washed 2 times the amount of resin volume by distilled water and 5 times the amount of resin volume by 0.12 mol·L-1 of HCl solution.Under those conditions,more than 60% extract was reduced with the proportion of total alkaloid in excess of 80%,and 70% of sample recover.Conclusion：D151 resin is suitable for industrial production for excellent drug loading and purification.