RP-HPLC测定麸煨肉豆蔻中去氢二异丁香酚的含量

Determination of Dehydrodiisoeugenol in Nutmeg Roasted with Wheat Bran by RP-HPLC

目的:建立麸煨肉豆蔻饮片中去氢二异丁香酚的含量测定方法。方法:采用高效液相色谱法,以甲醇-水(75∶25)为流动相,柱温为室温,检测波长为274 nm。结果:去氢二异丁香酚的进样量在0.075～0.60μg呈现良好现性关系(r=0.999 9),平均回收率为98.22%(n=6),RSD为1.9%。结论:采用高效液相色谱法测定麸煨肉豆蔻饮片中去氢二异丁香酚的含量,方法简便、灵敏、准确,可用于麸煨肉豆蔻饮片的质量控制。

Objective：To establish HPLC for determining the content of dehydrodiisoeugenol in Nutmegroasted with wheat bran.Method：An HPLC method was developed.The chromatographic conditions were asfollows：Diamonsil C18 column and methanol-water（75：25） as mobile phase,detection wavelength at 274 nm.Result：The linear range of dehydrodiisoeugenol was 0.075-0.60 μg（r=0.999 9）.The average recovery rate was 98.22% and RSD was 1.9%.Conclusion：The method is simple and accurate with good repeatability,and can be used for determination of dehydrodiisoeugenol in Nutmeg roasted with wheat bran.