HPLC测定丹红注射液中迷迭香酸在大鼠血浆中的浓度

Determnation of Concentration of Rosmarinic Acid of Danhong Injecta in Rat Plasma by HPLC

目的:建立丹红注射液中迷迭香酸在大鼠血浆中浓度的测定方法。方法:雄性SD大鼠6只,静脉注射丹红注射液(10 mL.kg-1)后,分别于不同时间取血,以肉桂酸为内标,液液萃取处理后,HPLC测定浓度。Diamonsil(钻石)C18色谱柱(4.6 mm×250 mm,5μm),乙腈-0.4%的磷酸水为流动相,梯度洗脱,流速1.0 min.mL-1,检测波长286 nm。结果:迷迭香酸在4.0～100.0 mg.L-1呈良好线性关系(R2=0.996 5);回收率在85.0%～101.4%,方法的日内、日间精密度(RSD)分别为4.00%～6.76%,4.50%～7.81%。不同时间点血浆中迷迭香酸的质量浓度分别为85.44,75.09,61.13,54.73,43.02,20.92,10.12,7.18,4.28 mg.L-1。结论:建立的方法灵敏度好、准确率高,可用于丹红注射液中迷迭香酸的药代动力学研究。

Objective：To establish a method for the determination of rosmarinic acid in rat plasma after iv Danhong Zhusheye.Method：Male SD rats six,after iv Danhong Zhusheye（10 mL.kg-1）,Separately at differ enttimes take blood,with cinnamic acid as internal standard,Liquid-liquid extraction processing,determination of rosmarinic acid by HPLC,Diamonsil C18 colurme was used.Acetonitrile-0.4% phosphoric acid solution as mobilephase,gradient elution,at the flow rate of 1 min.mL-1 with detection wave length at 286 nm.Result：Rosmarinic acid had good linear relationship when its concentration was between 4.0-100.0 g.L-1.Recoveries were between 85.0%-101.4%.The intra-day and inter-day precision of this method were 4.00%-6.76% and 4.50%-7.81%,respectively.Concentration of rosmarinic acid in rat plasma at different times Separately were 85.44,75.09 g.mL-1,61.13,54.73,43.02,20.92,10.12,7.18,4.28 mg.L-1.Conclusion ：The method is simple,quickand convenient for the determination of rosmarinic acid.