单向灌流法研究叶黄素微囊的大鼠在体肠吸收

Study on Intestinal Absorption of Lutein Microscapsules in Rats by in situ Single Pass Perfusion Method

目的:研究叶黄素微囊在大鼠在体肠吸收特性。方法:采用单向灌流模型,紫外分光光度法测定在体肠灌流叶黄素微囊质量浓度的变化,研究不同肠段、不同质量浓度叶黄素微囊的吸收部位和吸收机制。结果:叶黄素微囊在大鼠各肠段的吸收速率常数(Ka)、有效渗透系数(Papp)是回肠>空肠>十二指肠>结肠,且各肠段的Ka,Papp值无显著性差异;灌流液中不同质量浓度叶黄素微囊的Ka,Papp值均无显著性差异。结论:叶黄素微囊在大鼠肠道无特定吸收窗,药物质量浓度对叶黄素微囊的Ka,Papp值无影响,其吸收机制为被动扩散。

Objective：To study the in situ intestinal absorption behaviors of lutein microscapsules in rats.Method：In situ the rat single-pass intestinal perfusion model was used and the concentrations of the perfusate were determined by UV to investagate the intestinal absorption site and mechanism.Result：The Ka and Papp of Lutein Microscapsules showed ileumjejunumduodenumcolon in four different region of rat intestine.The Ka and Papp between four intestinal segments had no significant influence.The Ka and Papp of different drug concentration had no significant influence.Conclusion：Lutein Microscapsules could be absorbed in whole intestinal segment,drug concentration had no effect to Ka and Papp,its absorbing mechanism may be for passive diffusion.