厚朴酚对MPTP诱导PC12细胞凋亡的抑制作用

Inhibitive Effect of Magnolol Against MPTP-Induced Apoptosis in PC12 Cells

目的:探讨厚朴酚(mangolol,Mag)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导PC12细胞凋亡的抑制作用及可能作用机制。方法:将厚朴酚(终浓度0.1,10μmol.L-1)或/和MPTP(终浓度50,100,150,200μmol.L-1)加入到培养的PC12细胞中。四甲基偶氮唑盐法(MTT法)检测细胞增殖活性,用流式细胞仪检测细胞凋亡率,以及Western-blot法检测Bax和Bcl-2的蛋白表达变化。结果:在加入不同浓度的MPTP处理细胞24 h,细胞增殖活性渐次降低,而厚朴酚0.1～10μmol.L-1预处理1 h可明显减轻MPTP导致的PC12细胞的损伤,抑制细胞凋亡,以及Bcl-2/Bax比值的改变。结论:厚朴酚对MPTP诱导的PC12细胞凋亡损伤有一定的抑制作用,其作用机制涉及到影响细胞内Bcl-2/Bax蛋白的表达。

Objective：To investigate the inhibition of magnolol on the apoptosis of PC12 cell induced by MPTP and its mechanism.Method：Magnolol（final concentration 0.1,10 μmol·L-1） and /or MPTP（final concentration 50,100,150,200 μmol·L-1） were added into the culture of PC12 cells.The cell viability was detected with MTT method,apoptosis was measured by flow cytometry and Bcl-2/Bax ratio was detected by western blot.Result：Cell viability was declined sharply by MPTP with different concentrations,the pre-treatment of magnolol 0.1-10 μmol·L-1 could significantly increase the PC12 cell viability,apoptosis and the decrease in the Bcl-2/Bax ratio.Conclusion：Magnolol may inhibit the cell damage induced by MPTP and the mechanism is likely related to inhibition to expression of Bcl-2/Bax.