霉酚酸高产菌株短密青霉菌的选育

Selection for mycophenolic acid high-producing Penicillium brevicompactum

以短密青霉菌UH35-58为出发菌株,紫外线为诱变剂,采用摇瓶一级发酵补水工艺(64h补水20%)淘汰大量低产菌株,获得高产突变株N110-N76。用HPLC测定其发酵液的霉酚酸含量,发酵单位比出发菌株UH35-58提高120%。经过连续传代试验,该菌株的遗传性状稳定。

Objective To breed high producing strain of mycophenolic acid. Methods Penicillium brevicompactum UH35-58 was used as starting strain and treated by UV mutagenesis. The procedure, 20% water was fed after culturing 64 hours by flask-shaking fermentation, was adopted as preliminary screening to eliminate much of the low yield mutants. The titer of the culture was measured by HPLC method. Results and Conclusion A highyield mutant N110-N76 with the titer increased 120% was obtained from the parent strain UH35-58. The subculture test indicated that the hereditary character of strain N110-N76 was stable.