抗草丁膦转基因玉米不同DNA提取方法及PCR检测

Study on DNA Isolation Methods and PCR Detection of Transgenetic Maize Resistant to Glufosinae

以改良PEX,CTAB和SDS方法从转基因玉米材料中提取基因组DNA,并以外源CaMV35S启动子、bar基因及内源基因Zein的序列设计引物,用PCR方法扩增预期大小的DNA片段。结果显示,3种方法提取的DNA用以PCR检测都获得了预期大小的扩增片段,但改良PEX提取方法更省时省力。研究认为,改良PEX提取方法是一种简捷、快速和高效的植物DNA提取方法。

Using maize genomic DNA isolated from transgenic maize plants by PEX, CTAB and SDS methods, as a temples for PCR amplification, the primers were designed according to the sequences of CaMV35S promoter of exogenous gene, bar gene and endogenous gene Zein. The results of PCR amplification showed that 3 expected length fragments were amplificated from all genomic DNA that isolated by the above methods, however, the PEX method was less time and labor consuming. It is concluded that PEX method is a simple, fast and efficient method for plant genomic DNA extraction.