HPLC法测定香菇中香菇嘌呤含量

Determination of Eritadenine in Lentinula edodes Fruit bodies Using HPLC

建立1种运用高效液相色谱(HPLC)测定香菇(Lentinula edodes)中香菇嘌呤含量的方法。优化后的色谱条件为:Ultimate AQ-C18柱(5μm,4.6 mm×250 mm),流动相为甲醇-磷酸盐缓冲液(7∶93,pH 4.67)等度洗脱,流速1 mL/min,柱温30℃,检测波长259 nm,进样量10μL。研究结果发现该方法准确、灵敏、重现性好,适用于香菇嘌呤的定量分析。

A simple and sensitive HPLC-based method for the accurate determination of eritadenine in Lentinula edodes fruit bodies is described.Optimization of sample work-up procedures showed that the efficiency of eritadenine extraction was highest when powdered fruit bodies were suspended in 5%（w/v） ethanol and subjected to ultrasound for 30 min using a 1∶100 sample-solvent ratio.Optimal separation of eritadenine in the extracts was achieved by loading 10 μL samples on to an Ultimate AQ-C18 column（5 μm,4.6 mm×250 mm） and eluting with methanol-phosphate buffer（7∶93,pH 4.67） at 30 ℃ using a flow rate of 1.0 mL/min.Eritadenine in eluted fractions was monitored at 259 nm.