二维扩散排序核磁共振谱法研究β-环糊精/萘普生的相互作用

Study on the Interaction between β-Cyclodextrin and Naproxen by Two-dimensional Diffusion Ordered NMR Spectroscopy

采用二维扩散排序(DOSY)核磁共振法研究了萘普生与β-环糊精的相互作用,考察了不同包合因素对主客体自扩散系数及平衡常数的影响,并对萘普生与β-环糊精包合反应进行了热力学分析,以揭示萘普生与β-环糊精的包合反应机理。实验结果表明,温度、水含量、溶液pH值、离子强度等对客体萘普生的自扩散系数影响较大,但对主体环糊精自扩散系数的影响甚微;以上参数的增加均会使得平衡常数增大,有利于包合反应的进行,且在pH 9.0时包合作用最强;热力学参数进一步表明萘普生与环糊精的包合反应为自发吸热的熵驱动过程,主要作用为疏水力。

The interaction between naproxen and cyclodextrin was studied by two-dimensional diffusion ordered（2D-DOSY） NMR spectroscopy.Influences of different factors on the self-diffusion coefficient and the equilibrium constant were investigated.The results showed that the increase of temperature,water content,pH and ionic strength had more influence on the self-diffusion coefficient of the guest than that of the host,which could increase the equilibrium constants.The inclusion interaction was the strongest in a solution of pH 9.0.The results of thermodynamic analysis showed that the inclusion process was spontaneous and endothermic entropy-driven process,and the main active force was the hydrophobic force.