摘要
采用细菌学检测方法和PCR技术对来自四川省遂宁市某规模化养猪场疑似副猪嗜血杆菌(HPS)感染的发病猪只肺脏等病料进行了HPS分离鉴定,结果分离出1株疑似HPS的细菌,经形态学、染色及培养特性检测、生化试验、血清型试验16 S rDNA序列分析,鉴定为4型HPS。动物致病性试验显示HPS分离株均具有较强致病性,药敏试验表明HPS分离株具有较强的耐药性,仅对阿米卡星、氨苄舒巴坦、复达欣、痢特灵敏感。
The samples of sick pigs with suspected HPS infection collected from a large-scale pig farm in Suining,Sichuan Province, were detected by using bacteriology methods and PCR One strain type 4 HPS was isolated and identified through morphology.dyeing.culture characteristics.biochemical test.sertun-type test and 16 sr DNA sequence a- nalysis. Animal pathogenie test indicated this HPS isolated with highly-pathogenicity, and drug susceptibility test showed the HPS isolates with strong drug-resistant, it was only sensitive to amikacin.arnonia-sulbactam, fortum and furaxone.
出处
《动物医学进展》
CSCD
北大核心
2011年第8期115-118,共4页
Progress In Veterinary Medicine
基金
教育部<长江学者和创新团队发展计划>创新团队项目(IRTO848)
四川农业大学双支计划
关键词
副猪嗜血杆菌
生化
鉴定
PCR
血清学试验
药敏试验
Haernophilus parasuis
biochemistry
identify
PCR
serum-type test
drug susceptibility test
