生长调节剂和培养方式对匍枝筋骨草生根及β-蜕皮激素量的影响

Effects of growth regulator and culture methods on rooting of Ajuga lobata and content of β-ecdysone

目的研究不同质量浓度吲哚丁酸(IBA)和萘乙酸(NAA)以及培养方式对匍枝筋骨草生长和β-蜕皮甾酮量的影响。方法以MS为基本培养基,添加IBA(0.5、1.0、2.0 mg/L)或NAA(0.3、0.6、0.9 mg/L)进行组织培养,HPLC法测定匍枝筋骨草中β-蜕皮甾酮量。并比较不同生长调节剂以及组织培养、水培不同培养方式下匍枝筋骨草生根情况与β-蜕皮甾酮量的变化。结果以MS为基本培养基,添加生长调节剂IBA0.5～1 mg/L时,适宜匍枝筋骨草生根。IBA质量浓度为2 mg/L时,匍枝筋骨草根中β-蜕皮甾酮量最高。组织培养条件下,匍枝筋骨草全株及根部的蜕皮激素量均达水培苗的2倍,实生苗的3倍。结论适宜适量浓度的IBA可以促进匍枝筋骨草根的生长,组织培养条件下,匍枝筋骨草根中β-蜕皮甾酮量最高。

Objective To study the effects of indole butyric acid（IBA） and NAA at different concentrations and culture methods on the growth of Ajuga lobata root and content of β-ecdysone.Methods Taking MS as basic medium supplemented with IBA（0.5,1.0,and 2.0 mg/L） or NAA（0.3,0.6,and 0.9 mg/L）,the tissue culture was carried out.HPLC was used to determinate the content of β-ecdysterone.Results The results showed that the roots could grew well on MS as basic medium supplemented with IBA 0.5—1 mg/L.The best culture condition for the highest β-ecdysone accumulation was MS medium supplemented with IBA 2 mg/L.The content of β-ecdysterone in the whole plant and the roots of A.lobata with tissue culture was twice times of hydroponics and about three times of cultivation.Conclusion MS medium supplemented with IBA at the proper concentration could promote the root growth of A.lobata.The content of β-ecdysterone in the root of A.lobata is the highest in tissue cultures.