自分泌运动因子及受体mRNA表达与肝癌临床分期的关系

Relationship between AMF and AMFR mRNA level with clinical stages of liver cancer

目的探讨自分泌运动因子(AMF)、自分泌运动因子受体(AMFR)mRNA表达与肝癌临床分期的关系。方法对96例肝癌患者进行分期,采用逆转录实时荧光定量PCR测定患者肝癌组织中AMF、AMFR mR-NA表达。结果Ⅱ、Ⅲ期肝癌患者AMF、AMFR mRNA水平显著高于Ⅰ期,Ⅲ期肝癌患者AMF/AMFR mRNA水平显著高于Ⅱ期。肝癌转移组中AMF、AMFRmRNA表达明显高于未转移组。结论 AMF、AMFR mRNA水平与肝癌严重程度密切相关,可能对临床判断肝癌的严重程度具有一定的预测价值。

Objective To explore the relationship between AMF and AMFR mRNA level with the clinical stages of liver cancer. Methods 96 liver cancer sufferers were divided into three groups： the first stage,the second stage and the third stage groups. The expression of AMF and AMFR mRNA in cancer tissue was determined by realtime RT PCR method. Results The levels.of AMF and AMFR mRNA in the second stage and the third stage patients were significantly higher than those in the first stage group, and their expression in the third stage patients were sig- nificantly higher than that in the first stage group. The levels of AMF and AMFR mRNA in the of metastasis group were significantly higher than those in the non-metastasis group. Conclusion The levels of AMF and AMFR mRNA in cancer tissue are closely related with the severity of liver cancer,which maybe have some predictive value to judge the severity liver cancer in clinic.