基于SRAP标记的百合部分种及品种遗传结构分析

Genetic Relationship of Some Lilium Species and Cultivars Based on SRAP Marker

筛选了15对SRAP(Sequence-related Amplified Polymorphism)标记引物,对80个百合种及品种组成的样本进行标记,以所获的144个多态性位点和杂交试验获得的胚数,采用Pritchard分类方法对80个样本进行遗传结构分群分析。将试验样本划分为4个群,群体S1以亚洲百合杂种系的品种及原生种为主,群体S2以铁炮百合系的品种为主,群体S3以东方百合系的品种及原生种为主,群体S4则以OT杂交品种及原生种(S4)为主组成群,分群结果与传统百合杂种系的分类有一定的一致性;以S3群内部分样本杂交获得的胚数量作为亲和性指标与双亲群组分差异相关分析显示:群内双亲群组分差异绝对值与杂交获得胚的数量呈显著相关,表明分群及其组分能够比传统分类更好地体现出各样本间的主成分组成和遗传关系。据此,在百合杂交育种中可将分群以及组分作为亲本选配参考。

The genetic relationship of 80 Lilium species and cultivars was investigated at DNA level with SRAP（Sequence-related Amplified Polymorphism）marker.Fifteen polymorphic SRAP primers including 144 polymorphic loci were selected and hybrid embryo numbers was used to perform genetic structure analysis by Pritchard method.The total materials mentioned above were clustered into four main groups（gene pools） representing Asiatic hybrids and some wild species（S1）,Longiflorum hybrids（S2）,Oriental hybrids and others wild species（S3）,and Oriental × Trumpet hybrids（OT）and another wild species（S4）,which was consistent with the result of hybrids classification methods.Furthermore,genetic structure analysis revealed the pedigree between individuals better than traditional methods,and clarified the genetic relationship among species or cultivars.In addition,some cultivars from S3 groups as parents were used to hybridize,and significant correlations between the zygotic embryo numbers per 30flower buds obtained from each cross combination as one of the indexes in crossing compatibility and absolute value of difference between female parent and male parent in main estimated membership coefficients was revealed.This research will be useful to select parents in lily breeding.