秋水仙素诱导中间锦鸡儿多倍体的研究

Studies on Polyploid Induction of Caragana intermedia by Colchicine

以中间锦鸡儿(caragana intermedia)种子为试验材料,通过秋水仙素浸泡种子的方法研究了不同浓度及作用时间下秋水仙素对其染色体加倍的诱导效果。结果表明,直接浸泡种子,以0.3%秋水仙素浸泡48h为最佳,诱变率为53.3%;种子经萌发12h后再处理,以0.15%秋水仙素浸泡36h诱变率最高,为73.3%。对变异株根尖进行染色体计数后发现,二倍体对照2n=2x=16,变异株2n=3x=24为三倍体,变异株2n=4x=32为四倍体,同时也发现存在嵌合体现象。

Caragana intermedia were treated with different concentrations of colchicine solution at different stages in order to figure out the best inducing method and obtain polyploids. The results indicated that the mutation rate of seeds was 53.3% with the treatment of 0.3% colchicines for 48 h, while for sprouting seeds immersed in 0.15% colchicines for 36 h, the mutation rate was 73.3%. Chromosome observation found that the chromosome number of mutated plants was 2n = 4x = 32 （tetraploid） and 2n = 3x = 24 （triploid）, while that of the control plants was 2n = 2x = 16. The chimera was also found among treatment plants. A few polyploid seedlings were obtained in this study, this will provide the good base for Caragana polyploid breeding.