MAP-2在大鼠脑损伤后表达变化的时间规律性研究

An experimental study on expression of MAP-2 protein and mRNA after traumatic brain injury in rats

目的探讨MAP-2在大鼠脑损伤后表达变化的时间规律性。方法以355.09kPa冲击应力致大鼠局灶性脑损伤后,在不同时间应用原位杂交、免疫组化、免疫组化双染色技术检测MAP-2 mRNA和蛋白表达情况。结果 MAP-2mRNA在对照组及假手术组均有表达,伤后30 min表达增强,伤后2d,达到峰值,并维持高水平表达至伤后7d。MAP-2蛋白在对照组及假手术组均有表达,伤后3h开始呈下降趋势,伤后1d,达到最低值,伤后2d起,表达逐渐恢复,伤后15d,恢复至对照组水平。结论脑损伤后MAP-2 mRNA及蛋白表达具有时间规律,可用于法医学推断脑损伤形成时间。

Objective To investigate the relationship between the expression of MAP-2 and the time course of traumatic brain injury（TBI）.Methods The TBI Model was produced by the impact force of 355.09kPa.Immunocytochemical staining,doublelabeled staining,in situ hybridization and computer image analysis were employed.Results The MAP-2 mRNA and protein positive staining can be observed both in control and sham group.The MAP-2 mRNA positive staining remarkably increased from 30 minutes and reached the maximal level at 2 days after injury and kept at a high level for 7 days.The MAP-2 protein positive signals were observed in dendritic structure and cytosome.The positive dendritic structure staining decreased from 3h and reached the minimal level at 24h after injury.From 2 days after injury,the dendritic structure staining became to recover.There was no significant difference at 15 days after injury as compared with control group.Conclusion The expressions of MAP-2 mRNA and protein had a possible relationship with the extended periods of time after TBI.It might be used to determine time course of TBI in forensic medicine.