菠萝皮渣生产虾青素的发酵条件研究

Fermentation conditions optimization of the astaxanthin production for pineapple peel by Phaffia rhodozyma

以菠萝皮渣为培养基的主要原料,采用法夫酵母(Phaffia rhodozyma)对其进行发酵生产虾青素,采用响应面分析法对法夫酵母的发酵条件进行优化研究。先用Plackett-Burman设计法实验确定重要因素,再用最陡爬坡实验法确定因素水平,最后用响应面分析方法求得的最佳发酵条件为:发酵温度为20.0℃,初始pH为5.29,糖度为6.1%,酵母膏2g/L,MgSO42g/L,(NH4)2SO44g/L,KH2PO41.5g/L,种龄36h。用此优化的发酵培养基培养法夫酵母,虾青素产量可达6.5751mg/L。

Pineapple peel as main raw material was fermented with Phaffia rhodozyma to produce astaxanthin.Response surface analysis was applied to optimize the fermentation conditions for astaxanthin production.First,used Plackett-Burman design to select the most important fermentation conditions which affecting the growth of Phaffia rhodozyma.Then,the steepest ascent was used to approach region of the fermentation conditions.The optimal fermentation conditions were determined by a central composite design and response surface analysis.And the optimal conditions follows：temperature 20.0℃,initial pH 5.29,sugar content 6.1%,yeast extract 2g/L,MgSO4 2g/L,（NH4）2SO4 4g/L,KH2PO41.5g/L,seed age 36h.Using the optimal fermentation conditions to culture Phaffia rhodozyma,the yield of astaxanthin could be up to 6.5751mg/L.