多波长RP-HPLC测定山楂叶中芦丁、金丝桃苷及槲皮素含量

Simultaneous Determination of Rutin,Hyperoside,Quercetin in Folium Crataegi by Multiple wavelength RP-HPLC

目的:建立同时测定山楂叶中芦丁、金丝桃苷及槲皮素的分析方法。方法:采用多波长RP-HPLC法,色谱柱为Agilent Eclipse XDB-C18柱(4.6mm×150mm,5μm);流动相:乙腈(A)-0.025mol/L磷酸水(B),梯度洗脱依次为0～15min(18%A～18%A),15～30min(18%A～40%A);检测波长为363nm(0～18min),371nm(18～30min);流速:0.9mL/min;柱温:28℃。结果:芦丁、金丝桃苷及槲皮素的线性范围分别为0.3148～1.5740μg、0.4205～2.1024μg、0.0261～0.1305μg。平均加样回收率分别为100.29%、99.42%、98.86%,RSD分别为1.79%、1.46%、1.50%。结论:该方法简便、快速、准确,重现性好,可用于山楂叶中芦丁、金丝桃苷及槲皮素的同时测定。

AIM： To establish a method for simultaneous determination of rutin, hyperoside, quercetin in Folium Cra- taegi. Methods： A multiple wavelength RP- HPLC method was developed. The analysis was performed on an Agilent E- clipse XDB - C18column （4.6mm 150mm, 5μm）. A mixture of acetonitrile （A） - 0. 025mol/L phosphoric acid （B） with gradient elution as the mobile phase was adopted. The gradient elution program was 0 ~ 15min （18% A ~ 18% A）, 15 ~ 30min （ 18% A ~ 40% A） ; The wavelength was monitored 363nm （0 ~ 18min）, 371nm（ 18 - 30min）. The flow rate was 0.9 mL/min. The column temperature was 28 ℃. Results：The linear response ranges of rutin, hyperoside, quercetin were 0. 3148 - 1. 57401xg, 0. 4205 ~ 2. 1024μg, 0. 0261 - 0. 1305txg respectively. The average recoveries were 100.29%, 99.42%, 98.86% with RSD of 1.79%, 1.46%, 1.50% respectively. Conclusion： The method is simple and reproducible for determining rutin, hyperoside, quercetin in Folium Crataegi.