大黄降浊冲剂对5/6肾切除大鼠血清与胃窦组织胃泌素、血浆胃动素的影响

The Impact of Rhubarb Downbearing the Turbid Granules on 5/6 Nephrectomy Rat Serum and Antral Tissue Gastrin,Plasma Motilin

目的:研究5/6大鼠肾切除大鼠血清GAS、胃窦组织匀浆GAS及血浆MTL的水平变化及大黄降浊冲剂干预的影响。方法:以Wiatar雄性大鼠5/6肾切除的方法复制CRF大鼠模型。随机分为大剂量治疗组、小剂量治疗组[各ig大黄降浊冲剂1.0g/(kg.d)、3.0g/(kg.d)]对照组[ig尿毒清颗粒2.0g/(kg.d)],另设模型组、假手术组(各ig自来水),12周后比较观察各组间大鼠手术动物死亡率;24h尿蛋白定量;Tp、AIb、Hb;血SCr、BUN;测定血清GAS、胃窦组织匀浆GAS、血浆MTL。结果:治疗后对照组蛋白定量显著低于模型组(P<0.01),大、小剂量组SCr水平显著低于模型组(P<0.05、P<0.01)及对照组(P<0.01);治疗后假手术组与大剂量组血清GAS含量均显著低于模型组(P<0.01),假手术组及各用药组胃窦组织GAS含量均显著低于模型组(P<0.01),假手术组、小剂量组和对照组血浆MTL含量均显著低于模型组(P<0.05、P<0.01),而小剂量组降低显著(P<0.05)。结论:大黄降浊冲剂能改善高GAS血症,降低胃窦组织匀浆GAS水平,改善高MTL血症;具有降低肌酐、尿素氮,改善氮质血症,保护肾功能作用。

Objective ： To study 5 / 6 nephrectomy rat serum GAS, GAS and gastric tissue changes in plasma levels of MTL and Rhubarb Downbearing the turbid Granules interference. Methods ： Wiatar male rats 5 / 6 nephrectomy rat model of CRF method of reproduction. Randomly assigned to high - dose treatment group, low - dose treatment group [ all ig Rhubarb Downbearing the turbid Granules 1. Og/（ kg ·d）, 3.0g/（ kg · d） ] control group [ig Niaoduqing particles 2.0 g/（kg · d） ], a separate model group, sham operation group （ig water each）, compare observed after 12 weeks of surgery, rats in each group animal mortality; 24h urinary protein; T, A, Hb; serum Cr, BUN; serum GAS, gastric tissue homogenate GAS, plasma MTL. Results： After treatment, protein control group was significantly lower than model group （P 〈 0. 01 ） , large and small dose group Cr levels significantly lower than model group （ P 〈 0. 05, P 〈0.01 ） and the control group （P 〈 0. 01 ） ; treatment After the sham group GAS with large doses of serum levels were significantly lower than model group （ P 〈 0. 01 ）, sham group and the treatment group GAS gastric tissue levels were significantly lower than model group （ P 〈0.01 ）, the sham group , low -dose group and control group MTL plasma levels were significantly lower than model group （ P 〈 0. 05, P 〈0.01 ）, while the low dose group decreased significantly （ P 〈0.05）. Conclusion： Rhubarb Downbearing the turbid Granules to improve high - GAS hyperlipidemia, lower levels of gastric tissue GAS, improved high- MTL hyperlipidemia; with lower creatinine, urea nitrogen, improving azotemia, protect renal function.