益气补肾中药对自然流产模型小鼠NK细胞的影响

The Effect of the Traditional Chinese Medicine with Function of Invigorating Qi and Tonifying Kidney on Natural Killer Cells in Abortion-prone Mice

目的:观察益气补肾中药对自然流产模型小鼠脾脏、母胎界面组织NK细胞表达的影响。方法:以雌性CBA/J和雄性DBA/2为模型,孕0天始灌服益气补肾中药,并与环孢素A(CSA)对照,孕9天、14天流式分析脾脏、母胎界面NK细胞的表达。结果:孕9天各用药组NK细胞在脾脏及母胎界面的表达较阴性对照组明显降低(P<0.01或P<0.05);孕14天各用药组NK细胞在脾脏的表达较阴性对照组明显降低(P<0.05);孕14天中西医结合组NK细胞在母胎界面的表达较阴性对照组明显降低(P<0.05),而中药组、CSA组与阴性对照组比较无明显差异(P>0.05)。结论:益气补肾中药可下调自然流产模型小鼠孕9天及14天脾脏NK细胞表达及孕9天母胎界面NK细胞表达;益气补肾中药与CSA协同可下调自然流产模型小鼠孕14天母胎界面NK细胞表达,具有一定的免疫调节作用。

Objective ： To observe the effect of the traditional Chinese medicine （TCM） with tunction of invigorating Qi and tonifying kidney on the expression of natural killer （NK） cells at spleen and maternalfetal interface in abortion - prone mice during pregnancy. Methods： CBA female mice were mated to DBA /2 male mice as an abortion - prone model. Drenched the TCM decoction were made with function of invigorating Qi and tonifying kidney everyday since （lay 0 of gestation; And compared with Cyclosporin A. On the 9th and 14th day of gestation, the mice were killed in batches and their splenic and decidual tissues were made into single cell suspension to detect the expression of NK cells by FCM. Results： on the 9th day of gestation, the expressions of NK cells at spleen and deeidual tissues in all of lhe therapeutic groups were reduced obviously, comparing with the result in negative control group （ P 〈0.01 -0.05） ; on the 14th （lay of gestation, the expressions of NK cells at spleen in all of the therapeutic groups were reduced obviously, comparing with the result in negative control group（ P 〈 0.05 ） , and the expressions of NK cells at maternal - fetal interface, the TCM - WM intervention made the greatest promotion, having a significant difference with the negative control group （ P 〈 0.05 ） while the TCM group and CSA group had no obvious difference with the negative control group （P 〉 0. 05 ）. Conclusion： The traditional Chinese medicine with the function of invigorating Qi and tonifying kidney could decrease the expression of NK cells at spleen and early pregnancy of deeidual tissues in bortion - prone mice. The traditional Chinese medicine with function of invigorating Qi and tonifying kidney Combined with CSA could decrease the expression of NK cells at decidual tissues in middle and late gestaton of bortion - prone mice. Therefore the traditional Chinese medicine with function of invigorating Qi and tonifying kidney has certain immunomodulatory effects.