摘要
通过大孔树脂将注射液中2类成分分离,避免对测定结果的干扰。选用紫外-可见分光光度法分别测定2部分的黄酮和有机酸的含量。大孔树脂D101分离效果较好。以木犀草素-7-O-β-D-葡萄糖醛酸苷为对照品测得苦碟子注射液中总黄酮含量8.20mg/支,以菊苣酸为对照品测得苦碟子注射液中总有机酸含量为7.16mg/支。该分离方法重现性较好,2种测定方法准确,可靠,可以控制苦碟子注射液质量。
Macroporous resin was used for separating total flavonoids and total organic acids in Injection Kudiezi, in order to avoid interactive influence for determination of the results. The content of total flavonoids and total organic acids were delemined by spectrophotometry. Opyimum macroporous resin was D101. Luteolin-7-O-β-D-glucuronic acid was used as reference substance ,the content of total flavonoids was 8.20mg/per. Chicoric acid was used as reference substance ,the content of total organic acids was 7.16mg/per. Two methods were both simple, precise, accurate, and reliable for quality evaluation of Injection Kudiezi.
出处
《特产研究》
2011年第3期66-68,共3页
Special Wild Economic Animal and Plant Research
基金
吉林省中医药管理局项目(2010-137)
吉林省科技发展计划青年基金项目(201101132)
关键词
苦碟子注射液
总黄酮
总有机酸
大孔树脂
含量测定
Injection kudiezi
Total flavonoids
Total organic acids
Macroporous resin
Content
