白藜芦醇对大鼠局灶性脑缺血再灌注损伤的神经保护作用

Neuroprotective effect of resveratrol on focal cerebral ischemia-reperfusion injury in rats

目的:探讨白藜芦醇(resveratrol,Res)对大鼠局灶性脑缺血再灌注损伤的神经保护作用。方法:雄性SD大鼠150只,随机分为假手术组、模型组、低剂量组、中剂量组和高剂量组,每组30只。采用线栓法阻断大脑中动脉血供90 min,拔出栓线实现再灌注。缺血再灌注后1 h腹腔注射Res(低、中、高剂量分别为103、06、0 mg/kg)。再灌注后24 h,用2,3,5-氯化三苯基四氮唑染色显示脑梗死范围,并用干湿重法测定脑组织含水量;缺血再灌注后第7天,取大鼠脑组织固定包埋,HE染色。结果:Res各剂量组均可缩小脑梗死范围并改善大鼠的行为学障碍(P<0.05～P<0.01);Res各剂量组大鼠缺血侧大脑半球含水量均较模型组低(P<0.01);Res中剂量组可以改善脑缺血大鼠海马CA1区神经元损伤(P<0.05),但对齿回神经元形态无明显影响。结论:Res对局灶性脑缺血再灌注损伤具有神经保护作用,以Res 30 mg/kg治疗效果最好。

Objective：To explore the protective effects of resveratrol（Res） on focal cerebral ischemia-reperfusion injury in rats.Methods：One hundred and fifty Sprague-Dawley male rats were randomly divided into sham group,model group,low dose group,middle dose group and high dose group.Each group was 30 animals.The middle cerebral artery was occluded for 90 min by a nylon thread and then reperfused to cause transient focal cerebral ischemia.The rats were intraperitoneally injected with 3 doses of Res（10,30,60 mg/kg） about 1 h after ischemia-reperfusion as low,middle,and high dose group,respectively.Twenty-four hours after reperfusion,the infarct volume was showed with 2,3,5-triphenyl tetrazolium chloride staining.The water content was calculated with dry-wet weight method.Seven days after reperfusion,the brains of each group rats were cut in slices for HE staining.Results：The infarct volume and neurological score in each Res groups were lower than those in model group（P0.05-P0.01）.The water content in ischemia side brain in each Res groups was also lower than that in model group（P0.01）.The abnormal pyramidal neurons of hippocampus CA1 area in middle dose group were less than that in low dose group（P0.05）,but the neurons of dentate gyrus were no significant differences in each groups.Conclusions：The results suggest that Res has the neuroprotective effect on focal cerebral ischemia-reperfusion injury rats,and the 30 mg/kg dose of Res has the best therapeutic efficacy.