保护地土壤生防木霉菌种群多样性研究

Study on Genetic Diversity of Biocontrol Trichoderma Population in Greenhouse Soils

采用传统的形态学特征和分子方法(ITS、TEF序列和UP-PCR)研究了蔬菜保护地土壤中木霉菌种群多样性及其影响因素。结果表明:提交genbank 11个木霉种的序列,分别为长枝木霉(Trichoderma longibrachiatum)、拟康氏木霉(Trichoderma pseud-okoningii)、黄绿木霉(Trichoderma aureoviride)、棘孢木霉(Trichoderma asperellum)、深绿木霉(Trichoderma atroviride)、哈茨木霉(Tri-choderma harzianum)、非钩木霉(Trichoderma inhamatum)、微孢木霉(Trichoderma minutisporum)、长孢木霉(Trichoderma longipile)和粘绿木霉(Trichoderma virens);木霉菌24个菌株经UP-PCR扩增,引物AS4、AS19、L45扩增出一条500 bp大小的木霉菌种的特征性谱带,其他谱带则为多态性谱带,多态性达93.5%;营养条件、杀菌剂及土壤因素对不同种木霉菌的影响不同,得到2株适应性较强的木霉菌株,有望成为生防菌株。

11 Trichoderma species were submitted to genbank by ITS,TEF sequences analysis methods and effect factors of population diversity were studied in this paper, which included T. longibrachiatum, T. atroviride, T. harzianum, T. viren, T. minutisporum, T. pseudokoningii, Z aureoviride, T. irdramatum, T. asperellum, T. longipile and T. helicum. At the same time, genetic diverslty of Tricoderma isolates were analyzed by Universally Primed PCR. A feature hand ,500 bp, was the common one in 11 Trichoderma species,other bands were polymorphic,93.5 %. Futhermore, the effect of nutrition conditions, fungicides, soil factors etc. on Trichoderma was greatly different. 2 obtained strains which were strong adaptation ,would be good bio-control strains.