凉粉草组织培养快繁技术及优化研究(英文)

Rapid Propagation of Mesona chinensis Benth. in vitro and Its Optimization

以凉粉草带茎节的茎段为外植体,采用L9(34)正交设计,对影响凉粉草组培快繁过程的3个因素(6-BA、ZT、NAA)的3个浓度水平进行优化试验,试验结果采用SPSS13.0软件统计分析,并针对凉粉草在组培快繁过程中极易发生玻璃化等问题进行研究。结果表明:凉粉草的最适初代诱导培养基为MS+6-BA0.5 mg/L+NAA0.1 mg/L,无玻璃化;最适继代增殖培养基为MS+6-BA0.5 mg/L+ZT0.5 mg/L+NAA 0.02 mg/L+PVA 1000 mg/L,无玻璃化,增殖系数达9.1,芽壮;最适生根培养基为1/2MS+NAA0.1 mg/L+KT0.01 mg/L。1000 mg/L PVA为有效防治凉粉草组培快繁过程中玻璃苗发生的最佳浓度。采用这一技术,理论上1株试管苗一年可以生产凉粉草种苗约为9万株。

Aseptic nodal stem segments being used as explants,three factors （6-BA,ZT,NAA） affecting the in vitro rapid propagation of Mesona chinensis Benth. were optimized under three concentration levds by L9 （34 ） orthogonal design. The results showed that the optimal media for primary induction, subculture and rooting of M. chinensis Benth. in vitro were respectively as follows： MS ＋ 6 -BA 0.5 mg/L ＋ NAA 0. 1 mg/L （ no vitrification ）, MS ＋ 6-BA 0.5 mg/L ＋ ZT 0.5 mg/L ＋ NAA 0.02 mg/L ＋ PVA 100 mg/L （ resulted with high multiplication coefficient by 9.1, strong buds and no vitrification）, 1/2MS ＋ NAA0.1 mg/L ＋ KT0.01 mg/L. The suitable concentration of PVA used to avoid effectively the vitrification of tube-scedlings in rapid propagation of M. chinertsis Benth. was 1000 mg/L. By using this technique,theoretically,90 000 young individuals of M. chinensis Benth. would be produced from only 1 tube-seedling in one year. All experimental data were analyzed using SPSS 13.0. In addition, seedling vitrification during in vitro propagation was discussed also.