防霉剂对水霉菌的抑菌效果研究

Antifungal Effects of Antimoulds on Saprolegnia sp.

本试验研究了丙酸钙、山梨酸钾、双乙酸钠、脱氢乙酸钠、异噻唑啉酮、尼泊金乙酯等6种常用的防霉剂对水霉游动孢子及菌丝的体外抑菌效果。结果表明,异噻唑啉酮与尼泊金乙酯在浓度分别为1.5和6.3 mg/L以上时可使80%的水霉游动孢子的活性被抑制,当浓度分别为8和32 mg/L以上时对水霉菌丝具有杀灭作用。而其它四种防霉剂对水霉孢子的抑制浓度、对水霉菌丝的杀灭浓度远高于实际生产中可能的使用浓度。本试验还研究了异噻唑啉酮与尼泊金乙酯对水霉菌的后抑菌效应以及两者的联合抑菌效应,异噻唑啉酮在不低于6.25 mg/L,尼泊金乙酯在不低于25 mg/L时能够显著减缓水霉菌丝的生长速率,12.5 mg/L异噻唑啉酮和25 mg/L尼泊金乙酯还能显著改变菌丝的形态。异噻唑啉酮与尼泊金乙酯对水霉菌的抑制具有协同作用,其FIC指数为0.75。

The inhibition effects of 6 antimoulds on the Saprolegnia zoospore and mycelium in vitro were studied, including calcium propiohate, potassium sarbate, sodium diacetate, sodium acetate dehydrogenation, isothiazolone ketone and ethyl paraben. The results showed that the activity of 80 % of the Saprolegnia zoospore could be significantly inhibited by isothiazolone ketone and ethyl paraben above 1.5mg/L and 6.3 mg/L, respectively. Furthermore, the results aslo showed that the Saprolegnia mycelium could be killed by them above 8 and 32 mg/ L respectively. While the concentration of other four antimoulds which could inhibit the growth of Saprolegnia was much higher than the possible usa of actual production. This study also examined the later inhibitory effects to Saprolegnia mycelium of isathiazolone ketone and ethyl paraben and their combinational antifungal effect. The results showed that the growth of the mycelium could be significantly slowed down by them at the concentration above 6.25 and 25 mg/L, respectively. The configuration of mycelium could be significantly changed by them at the concentration above 12.5 and 25 mg/L, respectively, and they also had a synergistic inhibitory effect on Saprolegnia with, the FIC index of 0.75.