水稻钾离子通道基因OsKAT1.1的克隆、表达载体的构建及其电生理功能

Cloning,Construction of Expression Vectors and Electrophysiological Function of Potassium Channel Gene OsKAT1.1 in Rice

K+通道是植物高效吸收和体内运输K+的载体,对保证植物的钾素营养和增强抗逆性具有重要意义。水稻生长在淹水环境中,其K+通道在长期适应物种立地条件的进化过程中可能形成了有别于拟南芥等模式植物的独特功能特征和作用机制。本研究克隆了一个水稻KAT型K+通道基因OsKAT1.1,并利用电生理技术探讨其电生理功能。研究结果表明,通过一系列亚克隆过程,我们成功构建了适用于双电极电压钳和膜片钳电生理研究的表达载体pCI-OsKAT1.1和pTracer-CMV3-OsKAT1.1。进一步的电生理试验结果验证了构建过程的准确性,并表明水稻OsKAT1.1是一个由膜电位控制的吸收型K+通道。

As one of major mechanisms mediating K^＋ acquisition and redistribution in plants, K＋ channels are key machineries in supporting potassium nutrition as well as improving stress resistance. Growing in flooding paddy soil, rice K＋ channels could form preferential functional characteristics and regulatory mechanisms adaptive to the circumstances that are different from their counterparts found in dry land model plants such as Arabidopsis thaliana. However, our knowledge on rice K＋ channels is not well documented. Therefore, in this report, a KAT-type rice K＋ channel gene OsKA T1.1 was cloned and functionally studied through electrophysiological approaches. Expression vectors pCI-OsKATI.1 and pTracer-CMV3-OsKAT1.1 were successfully constructed for downstream studies with two-electrode voltage-clamp or patch-clamp respectively. Pilot electrophysiological measurements confirmed correct expression of target channel in both vectors, and proved that OsKATI.1 was a volt- age-gated K＋ uptake channel.