国内外鼠疫引物设计研究进展

On the advance of domestic and abroad plague primer design

目的研究国内外鼠疫PCR引物,了解引物设计种类及特征。方法收集各文献资料中PCR引物设计方法,进行整理归类。结果显示PCR引物设计按数量可分为单引物PCR、双引物PCR、多引物PCR。结论掌握PCR引物设计方法,同时采取相应的防假阴性、假阳性及UDPE防污染措施,可有效地将PCR应用于鼠疫检测中。

Objective To study about the domestic and abroad PCR plague primer and learn about the classification and features. Methods Different methods of the PCR plague primer designing in various literatures and materials are collected and categorized. Results PCR primer design can be classified into single primer PCR, double primers PCR and multiple primers PCR by its quantity. Qonclusion Mastery of the designing methods of PCR primers and relevant measures taken to prevent false negatives, false positives and UDPE contamination will be effectively help apply the PCR in the test of plague.