紫外诱变热带微藻选育高油脂藻株

Breeding of Lipid-rich Microalgae with Tropical Microalgae by Ultraviolet Mutation

为选育高含油量微藻藻种,本试验以优势藻株La4-37为原材料,采用紫外线辐射法对其进行诱变处理,获得了296株藻株。利用尼罗红荧光检测法对获得的藻株进行荧光检测,筛选获得相对含油量最大的诱变藻株M077和M040。通过对诱变株生长及脂荧光强度动态跟踪发现,诱变株生长周期和油脂积累时期基本一样,当达到平稳期时,诱变株油脂积累能力均有较大提高,脂荧光强度分别是原始藻株的6.2倍和1.7倍。

In order to obtain algal strains with higher oil content,La4-37 was treated with ultraviolet irradiation.296 microalgae were obtained after preliminary screening.Two mutagenic microalgae M077 and M040 with relatively lipid content were isolated through the lipid fluorescence detection and the lipid fluorescence intensity at the same algal density by Nile Red fluorescence staining method.The growth and lipid dynamic fluorescence intensity of mutagenic microalgae was showed that the growth cycle of mutant strains and the period of lipid accumulation were the same.When the mutagenic microalgae grew to the stationary phase,the lipid accumulation had improved greatly（the lipid fluorescence intensity of M077 and M040 was 6.2 and 1.7 times as that of La4-37,respectively）.