超声萃取/气相色谱法测定麝香中的麝香酮含量

Muscone Content was Measured by Ultrasonic Extraction/Mass Spectrometry in Musk

利用气相色谱法-氢火焰离子化检测器(GC/FID)快速测定麝香中麝香酮含量。样品经乙醇-超声波提取、过滤后,直接利用GC/FID测定,外标法定量。实验测定麝香酮回收率大于95%;定量限为0.80μg.mL-1;校准曲线线性范围为0-320μg.mL-1,线性相关系数r为0.9995;三次高、中、低浓度(50、150、300μg.mL-1)测定峰面积RSD%分别为1.08、0.89、0.55。本实验表明,乙醇超声波提取-GC/FID测定麝香中的麝香酮含量是一种简单、快速、准确的方法。

Muscone content was quickly measured by gas chromatography/flame ionization detector （GC/FID） in musk.. Samples were extracted by ethanol-ultrasonic, and filtered, directly determined by GC/FID, Recovery rate of Museone was more than 95% in experiment, quantify was limited 0.80 u g·mL-1 ; calibration curve linear range was 0-320 u g·mL-1, Linear correlation coefficient （r） was 0.9995; Peak area RSD of which three high-lever, medium, low concentration respectively were 50, 150, 300 ug·mL-1 were respectively determined 1.08,0.89,0.55.The result was showed that Muscone content was determined by ethanol-ultrasonic extraction and GC/FID was a simple, rapid, accurate method.