桃小食心虫基因组DNA提取方法比较研究

Comparison on Methods for Extraction of Genomic DNA of Carposina niponensis

以桃小食心虫(Carposina niponensis Walsingham)为材料,分别采用CTAB法,冰KAc法,SDS-PK法和试剂盒法提取桃小食心虫的基因组DNA。通过电泳、紫外光谱分析和ISSR-PCR扩增等检测手段,比较分析了DNA纯度。结果表明:CTAB法和SDS-PK法提取的DNA质量明显优于冰KAc法和试剂盒法,适用于PCR扩增。与其他方法比较,SDS-PK法提取的DNA经过PCR扩增后得到的多态性位点最多。因此,SDS-PK法是实验室提取桃小食心虫基因组有效、经济实用的方法。

Genomics DNA were extracted from Carposina niponensis Walsingham using CTAB, KAc, SDS-PK, and genomic DNAkit method, respectively. DNA was detected by electrophoresis, UV and ISSR-PCR. The results suggested that, the quality of DNA samples extracted by CTAB method and SDS-PK method was obviously better and more suitable for PCR than that by the other two methods. In contrast with other methods, SDS-PK method presented the most polymorphic sites. Therefore, the SDS-PK method was the most effective, practical and economic method in the genomic DNA extraction of Carposina niponensis in laboratory.