睡眠剥夺对吗啡成瘾小鼠学习记忆能力的影响

Study on the effect of sleep deprivation morphine-addicted ICR mice in learning and memory

目的:观察睡眠剥夺对吗啡成瘾小鼠的空间学习记忆能力的影响。方法:ICR小鼠40只随机分为4组(n=10):生理盐水对照组、吗啡成瘾实验组、生理盐水+睡眠剥夺对照组、吗啡成瘾+睡眠剥夺实验组。小鼠递增注射吗啡7 d建立成瘾模型后,通过睡眠剥夺箱48 h建立睡眠剥夺模型,水迷宫实验观察其学习记忆的前后差异对比。结果:与单纯吗啡成瘾组及睡眠剥夺组相比,吗啡成瘾+睡眠剥夺组与睡眠剥夺小鼠水迷宫逃避潜伏期时间明显延长(P<0.05)。结论:睡眠剥夺加重吗啡成瘾ICR小鼠的学习记忆能力的损害作用。

Objective： To investigate the effects of sleep deprivation on morphine-addicted mice spatial learning and memory ability by water maze. Methods： 40 ICR mice were randomly divided into 4 groups（n=10） ：morphine-addicted group, saline control group, sleep deprivation and morphine-addicted group, sleep deprivation and saline control group. The model of morphine dependent mice was established by gradually increasing the doses of morphine by subcutaneous injection （from 5 mg· kg^-1 to 40 mg· kg^-1 , 5 days）. Experimental model of sleep deprivation was established by use of flow pot method. Learning ability of mice was tested with water maze. Results： Compared with morphine-addicted group, sleep deprivation and saline control group, sleep deprivation and morphine-addicted group displayed much longer water maze escape latency time （P〈0. 05）. Conclusion： Sleep deprivation has an effect on morphine addiction and morphine withdrawal in mice.