高效阴离子色谱法测定卷烟主流烟气中的单糖

Analysis of monosaccharide in mainstream cigarette smoke by high performance anion exchange chromatography

建立了高效阴离子色谱．脉冲安培检测法同时检测卷烟主流烟气粒相物中6种单糖的方法。以蒸馏水为萃取剂，采用超声萃取吸附卷烟烟气总粒相物的剑桥滤片，经在线超滤后，以2．0mmol／LNaOH和0．5mmol／LNaAc的混合水溶液淋洗，采用积分脉冲安培检测，在METROSEP Carbl 250分离柱（4．6mm×250mm，5μm）上，卷烟主流烟气总粒相物中含有的果糖、葡萄糖、阿拉伯糖、甘露糖、半乳糖、木糖共6种单糖达到良好分离。结果表明：方法的重复性较好，6种单糖相对标准偏差〈4．31％；具有较高的灵敏度：6种单糖的检测限均低于0．10μg／支；较高的准确性：6种单糖的回收率均在85．10％-95．52％之间。

Analytical method of 6 monosaccharides in cigarette mainstream smoke was developed using high performance anionexchange chromatography （HPAEC） coupled with pulsed amperometric （amprerometric） detector. Total particulate matter of mainstream cigarette smoke was collected on Cambridge filter pad and extracted in distilled water by ultrasonic bath. Separation was accomplished on a METROSEP Carbl （4.6mm×250mm,5μm） separation column by using elution consisting of solution of 2.0mmol/L NaOH and 0.5mmol/L NaAc as mobile phase at a flow rate of lmL/min. Under these conditions, fructose, glucose, arabinose, mannose, galactose, and xylose in TPM were separated. Sugars were detected with amprerometric detector. Excellent reproducibility was achieved with RSDs of 6 monosaccharides below 4.31%. Limit of detection for every monosaccharide compound was less than 0.10μg/cig. The recoveries were in the range of 85.10% to 95.52%.