高效液相色谱法测定对乙酰氨基酚颗粒的含量

Determination of paracetamol granules by HPLC

目的建立对乙酰氨基酚颗粒含量的高效液相测定方法。方法色谱柱为Waters Sunfire CI8柱(4.6 mm×150 mm,5μm),流动相为磷酸盐缓冲液(pH4.5)-甲醇(80∶20),流速为1.0 ml.min-1,检测波长为254 nm,柱温为30℃。结果对乙酰氨基酚在0.05～0.40 g.L-1浓度范围内呈良好线性关系(r=1.0),平均加样回收率为99.9%(n=5),RSD为0.59%。结论该方法准确、可靠,可用于对乙酰氨基酚颗粒的含量测定。

Aim To establish an HPLC method for the determination of paracetamol granules.Methods Waters Sunfire C_I8 column（4.6 mm×150 mm,5 μm）was used with phosphate buffering solution（pH 4.5）-methanol（80∶20）as the mobile phase.The flow rate was 1.0 mL·min-1.The UV detection wavelength was 254 nm,and the column temperature was 30℃.Results The calibration curve of paracetamol was linear in the range of 0.05~0.40 g·L-1（r=1.0）.The average recovery（n=5）was 99.9% and RSD was 0.59%.Conclusion The method is accurate,reliable,and can be used for the determination of paracetamol granules.