摘要
目的建立测定毛鸡骨草药材中芹菜素-6,8-C-二葡糖苷含量的方法。方法用反相高效液相色谱法,色谱柱为Lichrospher C18(250 mm×4.6 mm,5μm);流动相为甲醇-0.1%三氟乙酸(35:65),流速1.0 mL/min,检测波长272 nm,柱温为室温。结果芹菜素-6,8-C-二葡糖苷在0.010 2～0.102 0 mg/mL范围内线性关系良好(r=0.999 8);平均回收率(n=6)99.3%,RSD 2.43%。结论所建方法简捷、快速,结果准确,可为评价毛鸡骨草药材的质量提供可靠的依据。
Objective To establish a method for the determination of 6,8-di-C-glucosylapigenin in Abrus mollis. Methods A RP-HPLC method was adopted. Chromatographic assay was performed on a Lichrospher C18 column （250 mm ×4.6 mm, 5μm） at room temperature with methanol - 0.1% TFA （35：65） as the mobile phase. The flow rate was 1.0 mL/min and the detection wavelength was 272 nm. Results The 6,8-di-C-glucosylapigenin had a good linearity in the range of 0.010 2-0.102 0 mg/mL（r = 0.999 8）. The average recovery was 99.3 % （n = 6） and RSD was 2.43 %. Conclusion The method is simple, accurate and reproducible. It can be used for the quality control ofAbrus mollis.
出处
《食品与药品》
CAS
2011年第9期337-340,共4页
Food and Drug
基金
广西科学研究与技术开发计划项目(桂科能05112001-3A5)
