胰腺癌细胞株Runx3基因启动子区CpG岛甲基化的检测

Detection of CpG island methylation of Runx3 gene promoter region in pancreatic cancer cell lines

目的探讨胰腺癌细胞株中Runx3基因启动子区CpG岛的甲基化水平。方法应用甲基化特异性PCR方法检测Panc-1、BxPC-3、AsPC-1三种胰腺癌细胞株及正常胰腺组织RunX3基因启动子区CpG岛甲基化水平。结果在Panc-1、BxPC-3、AsPC-1细胞株中Runx3基因启动子区CpG岛的甲基化引物均发生扩增,非甲基化引物未发生扩增;在正常胰腺组织中Runx3基因启动子区CpG岛的甲基化引物未发生扩增,非甲基化引物发生扩增。结论 Panc-1、BxPC-3、AsPC-1中存在Runx3基因启动子区CpG岛的完全甲基化。

Objective To identify CpG island methylation of Runx3 gene promoter region in pancreatic cancer cell lines. Methods CpG island methylation of Runx3 gene promoter region was determined by methylation-specific PCR in Panc-1, BxPC-3, AsPC-1 cell lines and normal pancreatic tissue. Results The methylated primers of CpG island of Runx3 gene promoter region other than non-methylated primers were amplified in Panc-1, BxPC-3 and AsPC-1 cell lines, while they were just the opposite in normal pancreatic tissue. Conclusion There exists the CpG island methylation of Runx3 gene promoter region in pancreatic cancer cell lines Panc-1, BxPC-3 and AsPC-1.