细胞角蛋白免疫组化染色对胃癌淋巴结转移的诊断意义

Diagnostic signficance of immunohistochemical cytokeratin staining on lymph node metastasis of gastric cancer

目的探讨细胞角蛋白(CK)免疫组化染色在判断胃癌淋巴结转移中的作用。方法采用常规HE染色、CK免疫组化染色检测51例胃癌根治术患者的淋巴结转移情况。结果 51例胃癌中,HE、CK免疫组化染色发现淋巴结转移检出率分别为58.8%、80.3%;在送检的848个淋巴结中,HE、CK免疫组化染色显示淋巴结转移率分别为12.3%、23.9%;两种方法的检出率和淋巴结转移率差异有统计学意义(P<0.01或0.05)。CK免疫组化染色的淋巴结转移检出率与胃癌分化呈负相关(r=-0.335,P<0.05)。结论 CK免疫组化染色可明显提高胃癌淋巴结转移的检出率,对判断胃癌分化和指导治疗有重要的临床意义。

Objective To explore the role of immunohistochemical cytokeratin（CK） staining in determining lymph node metastasis of gastric cancer.Methods Hematoxylin and eosin（HE） stain and immunohistochemical CK staining were used to detect lymph node metastasis in 51 patients with gastric cancer.Results HE and CK stain showed that the detection rates of lymph node metastasis were 58.8% and 80.3% in 51 cases,and metastatic rates of lymph node were 12.3% and 23.9% in 848 tested lymph nodes,respectively.There were significant differences between two methods（P〈0.01 or 0.05）.Lymph node metastasis determined by CK stain was negatively correlated with tumor differentiation（r=-0.335,P〈0.05）.Conclusion Immu-nohistochemical CK stain can improve the detection rate of lymph node metastasis in gastric cancer,which plays an important role in judging tumor differentiation and guiding therapy.