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CCL21/CCR7轴对人肺癌A549细胞VEGF-C表达影响的研究

A study of the effect of CCL21/CCR7 axis on VEGF-C expression in human lung adenocarcinoma A549 cells
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摘要 目的研究二级淋巴组织趋化因子/CC趋化因子受体7(CCL21/CCR7)轴对肺癌A549细胞血管内皮细胞生长因子-C(VEGF-C)表达的影响。方法实时定量PCR及Western Blot法检测CCL21作用前后A549细胞及A549-CCR7细胞VEGF-C mRNA及蛋白的表达。结果 CCL21作用下无论是在mRNA水平还是蛋白水平A549-CCR7细胞VEGF-C的表达均较A549细胞高。结论 CCL21与其受体CCR7结合能够促进A549细胞VEGF-C的表达,CCL21/CCR7轴可能参与了肺癌淋巴结转移的过程。进一步研究CCL21/CCR7轴和VEGF-C的关系可能有助于阐明肺癌淋巴结转移的机制。 Objective To investigate the effects of CCL21/CCR7 axis on VEGF - C expression of human lung adenocarcinoma A549 cells and 3.549 - CCR7 cells. Methods VEGF - C expression was detected using Real - Time RT - PCR and Western Blot in A549 cells and A549 - CCR7 cells before and after incubation with CCL21. Results Under the influence of CCL21, the VEGF - C expression of the A.549 - CCR7 ceils, in mRNA and protein levels, was significantly increased compared to that of A.549 cells （ P 〈 0.01 ）. Conclusion These data suggest that the bind of CCL21 to its receptor CCR7 leads to the increase of the VEGF - C expression of A549 cells, and CCL21/CCR7 axis may play a role in lymph node metastasis of lung cancer. To further study the relationship between CCL21/CCR7 axis and VEGF - C may help to elucidate the mechanism of lymph node metastasis in human lung cancer.
作者 郭学光 陈正堂 刘长庭
机构地区 中国人民解放军总医院南楼呼吸科 第三军医大学新桥医院全军肿瘤研究所
出处 《临床和实验医学杂志》 2011年第17期1313-1315,共3页 Journal of Clinical and Experimental Medicine
关键词 肺癌A549细胞CCL21 CCR7 VEGF—C淋巴结转移 Lung cancer A549 cell CCL21 CCR7 VEGF - C Lymph node metastasis
分类号 R734.2 [医药卫生—肿瘤]
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参考文献19

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二级参考文献16

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临床和实验医学杂志
2011年 第17期

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