hTERC基因在宫颈腺癌中的表达及临床意义

ponent gene in cervical adenocarcinomas

目的检测人类染色体端粒酶基因（hTERC）在宫颈腺癌组织中的表达情况，并进一步探讨hTERC基因在宫颈腺癌中异常扩增的临床意义。方法回顾性分析2000—2010年我院收治的15例宫颈腺癌患者的临床资料。应用荧光原位杂交技术（FISH）检测15例患者存档的病理石蜡切片标本中hTERC基因的扩增情况。同时取15例慢性宫颈炎组织作为正常对照，FISH检测其病理石蜡切片标本中hTERC基因的扩增情况。结果15例腺癌患者中有4例高危HPV病毒检测阴性。FISH检测15例宫颈腺癌的石蜡标本中的3号染色体hTERC基因15例均呈阳性表达，异常扩增阳性率为100％。宫颈炎对照组中未检测到hTERC基因异常扩增，异常扩增阳性率为0％。两组hTERC基因异常扩增阳性率相比差异有极显著性（尸〈0．01）。结论hTERC基因在宫颈腺癌组织中异常扩增，应用FISH技术检测宫颈腺癌中hTERC基因的表达，有助于临床早期诊断宫颈腺癌。

Objective To detect the expression and clinical significance of amplification of the human telomerase component（hTERC） in the specimens of cervical adenocarcinomas and discuss the clinical significance of its normal amplification. Methods Retrospective analysis was carried out to the clinical material of 15 cervical adenocarcinomaspatients who received operation in our hospital from 2000 to 2010. Their paraffin-embedded cervical tissues were established as experimental group,15 cervical speci- mens of chronic cervicitis women were established as control group. All of specimens were detected by FISH to evaluate the hTERC gene amplification. Results No control group samples revealed copy number increases of 3q, while 100 % of cervical adenocarcinomas tissues showed extra copies of 3q. hTERC copy numbers in cervical adenocarcinomas were significantly higher than those in control group I samples, （_P〈0.001）. Conclusions hTERC gene showed abnormal amplification in the cervical adenocarcinomas. And the detection of hTERC gene amplification by FISH could help the early diagnosis of cervical adenocarcinomas.