鹅细小病毒荧光定量PCR检测方法的建立及应用

Establishment and Application of Fluorescence Quantitative PCR for Identification of Goose Parvovirus

利用已经分离得到的小鹅瘟病毒GPV SP株,设计合成1对特异性的引物和Taq Man探针,通过特异性试验、敏感性试验建立了GPV Taq Man荧光定量PCR检测方法。同时饲养产蛋种鹅,接种小鹅瘟病毒,在不同的时间段采取病料,并且利用荧光定量PCR检测病毒的存在与含量,得到了小鹅瘟病毒在产蛋种鹅体内的定植规律。

In this study, the GPV SP strain was used to find out the selfish gene sequence of the GPV VP3 according to the GenBank. Then the fluorescent quantitative PCR method was developed for the detection of GPV based on the specific primer and the probe of Taq Man. The reaction system and reaction condition were optimized, and the standard curve was established to evaluate the specificity and sensitivity. In order to study the field planting disciplinarian of the GPV in the egg kind of goose, the GPV virus was inoculated to the goose, the disease materials were taken at the different times, and then the fluorescent quantitation PCR method was used to detect the existence and content of the virus.