中兽药散剂中非法添加利巴韦林的检测方法研究

Study on the Determination Method of Ribavirin Illegally Added in the Veterinary Traditional Chinese Herb Medicine Powders

建立了清瘟败毒散和苍术香连散两种中兽药散剂中违规添加利巴韦林的检测方法。样品经显微检查法初筛,对阳性样品进行高效液相色谱定量分析。结果显示,在1～150μg/mL浓度范围内线性关系良好;添加平均回收率在91.74%～98.50%之间,变异系数在0.08%～0.58%之间,检测限为1～2 mg/kg。该方法快速、准确,可用于上述两种中兽药散剂中违规添加利巴韦林的定量测定。

The determination method of ribavirin violated in the two veterinary traditional Chinese herb medicine powders including Qingwenbaidusan and Cangzhuxiangliansan was established. Preliminarily observed by microscope, the positive samples were determined by HPLC. The results showed that the standard curves for ribavirin were in good linearity within a concentration range of 1 - 150 μg/mL. The average recoveries for ribavirin at the addition in the two powders ranged from 91.74% to 98.50% with the RSD from 0.08% to 0.58%. The limit of quantification （LOQ） was 1 -2 mg/kg. This method was fast and accurate, and could be applied to determine ribavirin in the two veterinary traditional Chinese herb medicine powders.