多药耐药铜绿假单胞菌16S rRNA甲基化酶及氨基糖苷类修饰酶基因研究

Study on genes of 16S rRNA methylase and aminoglycoside-modifying enzymes in multidrug-resistant Pseudomonas aeruginosa

目的探讨多药耐药铜绿假单胞菌(MDRPA)氨基糖苷类耐药机制。方法应用PCR检测18种16SrRNA甲基化酶和氨基糖苷类修饰酶基因。结果 20株MDRPA检出aac(3)-Ⅱ、aac(6′)-Ⅰb、aac(6′)-Ⅱ、aph(3′)-Ⅱb等4种氨基糖苷类修饰酶基因,阳性率分别为60.0%、30.0%、60.0%、35.0%,共有12株检出氨基糖苷类修饰酶基因。结论该组MDRPA氨基糖苷类耐药机制与产氨基糖苷类修饰酶相关。

OBJECTIVE To investigate the mechanisms of aminoglycoside resistance in multidrug-resistant Pseudomonas aeruginosa（MDRPA）.METHODS Totally 18 genes of 16S rRNA methylase and aminoglycoside-modifying enzymes were detected by polymerase chain reaction（PCR） and verified by DNA sequencing.RESULTS In 20 strains of MDRPA,the positive rates of aac（3）-Ⅱ,aac（6′）-Ⅰb,aac（6′）-Ⅱ and aph（3′）-Ⅱb were 60.0%,30.0%,60.0% and 35.0%,respectively.Aminoglycoside-modifying genes were identified from 12 strains of MDRPA.CONCLUSION The aminoglycoside resistance mechanism of MDRPA is related to aminoglycoside-modifying enzymes.