日粮锌源和锌水平对断奶仔猪免疫功能和抗氧化酶活性的影响

Effects of zinc resources and levels in diets on immunity and activity of anti-oxidation enzyme of weanling piglets

选用21日龄"杜×长×大"三元杂交的断奶仔猪40头,按体质量和性别随机分成4组(Ⅰ～Ⅳ组),每组10头猪。分别饲喂添加氧化锌(以锌量计)Ⅰ组(对照组)100mg/kg、Ⅱ组3 000mg/kg和蛋氨酸锌(以锌量计)Ⅲ组100mg/kg、Ⅳ组300mg/kg相同的基础日粮,预试验7d,正式试验42d。结果显示:脾脏、胸腺指数Ⅱ组>Ⅳ组>Ⅲ组>Ⅰ组,但各组间差异均不显著(P>0.05);试验第14天,淋巴细胞转化率D值Ⅱ、Ⅳ组极显著高于Ⅰ组(P<0.01);试验第14、28、42天,猪瘟抗体水平Ⅱ、Ⅳ组极显著高于Ⅰ组(P<0.01);Ⅱ、Ⅳ组能提高血清Cu/Zn-SOD、CAT、GST-PX的活性,降低血清MDA含量,且在试验第14、28天时效果更好。结果表明,高锌日粮能显著提高断奶应激仔猪免疫功能和抗氧化酶活性。

The purpose of this experiment was to study the effects of different levels and kinds of zinc diets on the immunity and activity of anti-oxidation enzyme in serum of weanling stress piglets.Forty ＂Duroc×landrace×Yorkshire＂ crossbred weanling piglets（21 d of age）were randomly divided into 4 groups of 10 piglets each by weight and sex,which were fed a basal diet with 100 or 3 000 mg/kg zinc（from ZnO）,and 100 or 300 mg/kg zinc（from Zn-Met）,respectively.The trial lasted for 49 days,including preset period for 7 days and test period for 42 days.The results showed that：index of spleen and thymus in each group had no significant effects（P0.05）;lymphocyte transformation rate in groups Ⅱand Ⅲ was significantly increased compared with this group Ⅰ（P0.01） on the 14 th day of the experiment;the valence of CSFV antibody in groups Ⅱand Ⅳwas significantly increased compared with this group Ⅰ（P0.01） in total days of the experiment;serum activity of anti-oxidation enzyme（Cu/Zn-SOD,CAT,GST-PX） in groupsⅡand Ⅳ was increased compared with those in other groups,but serum MDA content was reduced.The obvious effect was observed on the 14 th and 28 th day of the experiment.It is confirmed that the high-level zinc diets could increase immunity and activity of anti-oxidation enzyme of weanling piglets with weanling stress.