葵花籽粕中提取绿原酸的工艺优化

Process Optimization of Extraction of Chlorogenic Acid in Sunflower Meal

研究了从葵花籽粕中提取绿原酸的工艺。通过对甲醇浓度、温度、料液比、提取时间等因素对提取效率的影响,在运用旋转蒸发仪下得出绿原酸提取工艺的最佳工艺参数:甲醇浓度70%、温度45℃、提取时间1.5 h、料液比1∶10(W/V)。采用紫外分光光度法对绿原酸产品进行检测。在325 nm波长下,得到绿原酸的最大峰值,计算得率为3.13%。

Extraction of chlorogentic acid from sunflower seed meal was studied systematically.The rotary evaporatr assisted extraction of chlorogenic acid,the effect of factors was investigated and the optimal parameters were determined as follows： the concentration of methanol was 70 %,the ratio of sunflower seed meal to methanol was 1∶10（W/V）,time was 1.5 h.The use of UV spectrophotometry for detection of chlorogenic acid products.In the 325 nm wavelength,obtained the maximum peak of chlorogenic acid,was 3.13 % calculated.