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阳离子脂质体基因转染研究

Study on Gene Transfection Mediated by Cationic Liposomes
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摘要 目的:评价阳离子脂质体介导基因转染宫颈癌细胞的转染效果,优化对宫颈癌细胞的转染条件。方法:以荧光素酶基因pGL3为报告基因,分析影响阳离子脂质体Lipofectamine 2000转染Hela效率的因素。结果:通过细胞转染实验,得出脂质体与pDNA的稀释液混合较佳等待时间为30 min,脂质体/pDNA复合物与细胞接触的转染时间为4 h,转染效率较高。结论:Lipofectamine2000 Hela细胞转染效率较高。 Objective： To evaluate the gene transfection efficiency of cationic liposome to cervical carcinoma cell,in addition,to optimize the transfection conditions.Method： the transfection efficiency of liposome were examined using Luceferase as reporter gene.Result： Lipofectamine 2000 could get higher transfection efficiency when dilutin time was 30 min before complexing and the optimum transfection time was 4 h.Conclusion： Lipofectamine 2000 obtained higher transfectin efficiency in Hela cells.
作者 韩维奇 关山飞 朴林波 梁超 张树彪 王冰
机构地区 大连民族学院生命科学学院
出处 《广东化工》 CAS 2011年第9期36-36,64,共2页 Guangdong Chemical Industry
关键词 阳离子脂质体 基因转染 报告基因 cationic liposome gene therapy reporter gene
分类号 TQ [化学工程]
  • 相关文献

参考文献6

  • 1Ma B, Zhang S, Jiang H, et al. Lipoplex morphologies and their influences on transfection efficiency in gene delivery[J]. J Control Release, 2007, 123: 184-194.
  • 2Zhang S, Xu Y, Wang B, et al. Cationic compounds used in lipoplexes and polyplexes for gone delivery[J], J Control Release, 2004, 100: 165-180.
  • 3Zhang S, Zhao B, Jiang H, et al.Cationic lipids and polymers mediated vectors for delivery ofsiRNA[J]. J Control Release, 2007, 123: 1-10.
  • 4Elouahabi A, Ruyssehaert J M..Formation and Intracellular Trafficking of Lipoplexes and Polyplexes[J]. Mol Ther, 2005, 11: 336-347.
  • 5段艳,王冰,崔韶辉,赵不凋,姜云霞,杨颖,姜慧明,张树彪.阳离子脂质体转染Hela细胞的实验[J].中国组织工程研究与临床康复,2009,13(11):2119-2122. 被引量：14
  • 6Dalby B, Cates S, Harris A, et al. Advanced transfection with Lipofectamine 2000 reagent : primary neurons , siRNA , and high-throughput applications[J]. Methods, 2004, 33: 95-103.

二级参考文献20

  • 1苏涛.绿荧光蛋白基因质粒表达载体对SACC-83的转染效率及瞬时表达特点[J].中国组织工程研究与临床康复,2007,11(6):1087-1090. 被引量：1
  • 2Cavazzana-calvo M, Thrashier A, Mavilio F. The future of gene therapy. Nature. 2004;427 (6977): 779-781.
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  • 5Zhang S, Zhao B, Jiang H,et al. Cationic lipids and polymers mediated vectors for delivery of siRNA. J Control Release. 2007;123:1-10.
  • 6Ma B, Zhang S, Jiang H,et al. Lipoplex morphologies and their influences on transfection efficiency in gene delivery. J Control Release. 2007;123:184-194.
  • 7Lv H, Zhang S, Wang B, et al. Toxicity of cationic lipids and cationic polymers in gene delivery. J Control Release. 2006:114(1):100-109.
  • 8Pari GS, Keown WA. Experimental strategies in efficient transformation of mammalian cells: calcium phosphate and DE-AE dextran. Methods Mol Biol. 1997; 62 (1): 301-306.
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  • 10Madry H, Reszka R, Bohlender J, et al. Efficacy of cationic liposome-mediated gene transfer to mesangial cells in vitro and in vivo. J Mol Med. 2001 ;79 (4): 184-189.

共引文献13

广东化工
2011年 第9期

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