病毒感染患者抗病毒治疗前后淋巴细胞亚群的变化及意义

The change and significance of peripheral blood lymphocyte subsets after antiviral therapy in patients with acute virus infection

目的通过对病毒感染急性期患者抗病毒治疗前后外周血淋巴细胞亚群的检测,比较其差异,初步探讨病毒感染患者细胞免疫功能的变化及抗病毒治疗对宿主细胞免疫功能的影响。方法收集2010～2011年感染科收治的病毒感染急性期患者20例,使用流式细胞仪检测其外周血T细胞亚群,与正常对照组比较,分析其差异。选取其中10例患者,于抗病毒治疗1周后再次检测外周血T细胞亚群,比较治疗前后变化并分析其意义。结果与正常对照相比,病毒感染患者外周血B细胞减少,T细胞、CD8+T及CD8+CD38+T细胞比率及计数升高。抗病毒治疗1周后,患者白细胞、CD8+CD38+T细胞计数降低。结论病毒感染急性期患者体内存在T淋巴细胞亚群失衡和细胞免疫功能紊乱,而早期抗病毒治疗有助于减轻病毒感染导致的异常免疫激活和免疫损伤。

Objective To investigate the change of host＇s cellular immunity and the effect of antiviral therapy on host＇s cellular immunity in patients with acute virus infection. Methods Flow cytometry was used to detect the peripheral blood lymphocyte subsets in 20 patients with acute virus infection and 20 health blood donors. And re - examine of peripheral blood lymphocyte subsets was performed in 10 patients after antiviral therapy. Results Compared with normal controls, the count and the proportion of lymphocytes, T - lymphocytes, CD8^＋ T cells and CD8 ＋ CD38^＋ T cells increased in patients with acute virus infection. And the count of leucocytes and CD8^＋ CD38^＋ T cells decreased after antivi- ral therapy for a week. Conclusion There was imbalance in peripheral blood lymphocyte subsets and turbulence in cellular immunity in patients with acute virus infection. And early antiviral therapy could relieve the abnormal immune activation and immune injury after virus infection.