HPLC法测定导赤丸中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量

Determination of Aloe-emodin, Rhein,Emodin,Chrysophanol and Physcion in Daochi Pills by HPLC

目的制定测定导赤丸中芦荟大黄酸、大黄素、大黄酚、大黄素和大黄素甲醚5种活性成分含量的方法。方法利用高效液相色谱法进行测定,采用流动相A:100%乙腈-0.1%磷酸,B:10%乙腈-0.1%磷酸以梯度浓度洗脱;流速控制在1mL/min;波长设定为254nm;柱温30℃。结果芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚质量浓度与峰面积均呈良好的线性关系,平均回收率分别为99.7%(RSD为1.28%n=5),99.1%(RSD为1.68%n=5),99.6%(RSD为0.90%n=5),98.8%(RSD为0.89%n=5),99.7%(RSD为1.04%n=5)。结论本法结果准确,操作简便,可靠,重复性好,可用于导赤丸的质量控制。

Objective To develop an HPLC method for simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Daochi Pills. Methods The sample was separated on a Shimadzu VP-ODS （4.6mm×250mm, 5.01.tin） with acetonitrile-0.1% phosphoric acid in gradient elution. The flow rate was 1.0 mL/min, the detection wavelength was 254 nm and the column temperature was 30℃. Results Their average recoveries were 99.7% （RSD =1.28%, n = 5）, 99.1% （RSD = 1.68%, n = 5）, 99.6% （RSD = 0.90%, n = 5）, 98.8% （RSD = 0.89%, n = 5）, and 99.2% （RSD = 0.99%, n = 5）, respectively. Conclusion The method is rapid, simple, accurate, and applicable for the quality control of Dahuang Zhechong pills.