摘要
目的建立测定宫环养血颗粒中芍药苷含量的HPLC方法。方法采用Shim—pack VP—ODS柱，乙腈-0．05％磷酸水溶液（16：84）为流动相，检测波长为230nm，流速1．0mL／min。结果芍药苷在0．0476-0．912μg范围内线性关系良好，r=0．9996，平均回收率为99．51％，RSD为1．35％（n=6）。结论该方法简便、准确、重现性好，可用于宫环养血颗粒的质量控制。
Objective To establish a HPLC for determination of Paeoniflorin in Gonghuan Yangxue Granule. Method A Shim-pack VP-ODS column was used. The mobile phase was acetonitrile-0.05%phosphate （ 16 ： 84） , the detecting wavelength was at 230nm, the flow rate was 1.0mL/min. Result A good linearity of Paeoniflorin was in the range of 0.0476-0.9121ag, r=0.9996, the average sample recovery was 99.51% with RSD of 1.35% （n=6） . Conclusion This method has good repeatability and flexibility. It can be used for quality control of Gonghuan Yangxue Granule.
出处
《中国医药指南》
2011年第26期33-33,35,共2页
Guide of China Medicine
