龙柏组织培养研究

Study on the Tissue Culture of Sabina chinensis cv.Kaizuka

[目的]为龙柏的快速繁殖提供参考。[方法]用圆柏(对照材料)、龙柏的当年生幼嫩茎段为外植体,以MS为基本培养基附加不同浓度的激素对其进行诱导分化和增殖研究,筛选诱导芽分化、增殖的最佳激素组合。[结果]接种前,将圆柏和龙柏的外植体用浓度75%乙醇浸泡30 m in,无菌水冲洗几次,用浓度0.2%HgC l2消毒5 m in,无菌水洗5次,然后置于培养皿上沥干,消毒效果最好。在1/2MS+6-BA 0.5 mg/L+NAA 0.5 mg/L的培养基上,圆柏试管苗分化率最高,生长良好;在1/2MS+6-BA 1.5 mg/L+NAA 0.5 mg/L的培养基上,龙柏试管苗分化率最高,生长良好。在MS+6-BA 1.0 mg/L+NAA 0.01 mg/L的培养基上,龙柏增殖效果最好。[结论]建立了龙柏组织培养体系。

[Objective] The reference for the rapid multiplication of Sabina chinensis cv.Kaizuka was provided through the experiment.[Method] The tender stem sections of Sabina chinensis（CK） and Sabina chinensis cv.Kaizuka,as explants,were cultured in the medium MS that was added with the different concentrations of hormone for the experiment in their induction and differentiation so that the best combination of hormone for budding induction and its multiplication was selected.[Result] The best sterilizing method of the tender stem sections of Sabina chinensis（CK） and Sabina chinensis cv.Kaizuka was as follows： the explants were soaked in 75% alcohol for 30 minutes and washed with aseptic water for many times,then,sterilized with 0.2% HgCl2 for 5 minutes and washed with aseptic water for 5 times,and finally,naturally dried in culture dish before their culturing.In the medium of 1/2MS＋6-BA 0.5 mg/L＋NAA 0.5 mg/L,Sabina chinensis grew well with the highest differentiation rate.In the medium of 1/2MS＋6-BA 1.5 mg/L＋NAA 0.5 mg/L,Sabina chinensis cv.Kaizuka grew well with the better differentiation rate.In the medium of MS＋6-BA1.0 mg/L＋NAA 0.01 mg/L the multiplication of Sabina chinensis cv.Kaizuka plantlet was best.[Conclusion] The system of rapid multiplication of Sabina chinensis cv.Kaizuka was established.